From Erlenmeyer flask to microplates – lessons learned from measuring Escherichia coli growth




E coli, growth curve, microplate


Growth curve measurements are fundamental operations in microbiology and molecular biology. Microplate readers have the advantage of high-throughput and automation. This allows for real-time monitoring of many bacterial cultures and a decrease in labor and costs. The volume and geometry of a well from a microplate imply that calibration and comparative studies must be performed before switching from one cultivation and growth monitoring system to another. Here, Escherichia coli XL1 Blue was used as a reference strain in order to test the feasibility of using a common, filter-based microplate reader for recording growth curves when performing antibiotic and toxicity testing. 


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2023-08-10 — Updated on 2023-10-11

How to Cite

Abalașei, M., Pioară, M., & Mihasan, M. (2023). From Erlenmeyer flask to microplates – lessons learned from measuring Escherichia coli growth. Journal of Experimental and Molecular Biology, 24(3), 145–150.



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