From Erlenmeyer flask to microplates – lessons learned from measuring Escherichia coli growth

Marinela Abalașei; Mihaela Pioară; Marius Mihasan

doi:10.47743/jemb-2023-112

From Erlenmeyer flask to microplates – lessons learned from measuring Escherichia coli growth

Authors

Marinela Abalașei
Mihaela Pioară
Marius Mihasan Alexandru Ioan Cuza University of Iasi http://orcid.org/0000-0003-4439-4251

DOI:

https://doi.org/10.47743/jemb-2023-112

Keywords:

E coli, growth curve, microplate

Abstract

Growth curve measurements are fundamental operations in microbiology and molecular biology. Microplate readers have the advantage of high-throughput and automation. This allows for real-time monitoring of many bacterial cultures and a decrease in labor and costs. The volume and geometry of a well from a microplate imply that calibration and comparative studies must be performed before switching from one cultivation and growth monitoring system to another. Here, Escherichia coli XL1 Blue was used as a reference strain in order to test the feasibility of using a common, filter-based microplate reader for recording growth curves when performing antibiotic and toxicity testing.

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Published

2023-08-10 — Updated on 2023-10-11

How to Cite

Abalașei, M., Pioară, M., & Mihasan, M. (2023). From Erlenmeyer flask to microplates – lessons learned from measuring Escherichia coli growth. Journal of Experimental and Molecular Biology, 24(3), 145–150. https://doi.org/10.47743/jemb-2023-112

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Issue

Vol. 24 No. 3 (2023)

Section

Short Communications

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