From Erlenmeyer flask to microplates – lessons learned from measuring Escherichia coli growth
DOI:
https://doi.org/10.47743/jemb-2023-112Keywords:
E coli, growth curve, microplateAbstract
Growth curve measurements are fundamental operations in microbiology and molecular biology. Microplate readers have the advantage of high-throughput and automation. This allows for real-time monitoring of many bacterial cultures and a decrease in labor and costs. The volume and geometry of a well from a microplate imply that calibration and comparative studies must be performed before switching from one cultivation and growth monitoring system to another. Here, Escherichia coli XL1 Blue was used as a reference strain in order to test the feasibility of using a common, filter-based microplate reader for recording growth curves when performing antibiotic and toxicity testing.
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Copyright (c) 2023 Marinela Abalașei, Mihaela Pioară, Marius Mihasan
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Ministerul Cercetării şi Inovării
Grant numbers PN-III-P4-ID-PCE-2020-0656