IN VITRO CYTOPHYSIOLOGICAL RESPONSE INDUCED BY THREE FOOD ADDITIVES ON SOME MAMMALIAN CELL LINE MODELS

Daniela Gherghel; Cosmin-Teodor Mihai; Gabriela Vochita; Craita Maria Rosu

doi:10.47743/jemb-2024-149

Authors

Daniela Gherghel Department of Experimental and Applied Biology
Cosmin-Teodor Mihai Department of Experimental and Applied Biology
Gabriela Vochita Institute of Biological Research Iasi, branch of NIRDBS
Craita Maria Rosu

DOI:

https://doi.org/10.47743/jemb-2024-149

Keywords:

food additives, MTT assay, cell morphology test, clonogenesis assay

Abstract

This research is focused on cellular response to sodium metabisulphite (E223), sodium benzoate (E211), and sodium nitrite (E250) application on two normal mammalian cells, namely MCF-12A (ATCC CRL-3598) and Vero (ATCC CCL-81). The monitored parameters were cell viability (MTT assay), cell morphology (optical microscopy) and cell survival (clonogenic assay). The treatment was applied in doses of 12.5; 25; 50 and 100 µg/mL. According to all tests assessed, our results proved a dose-response relationship, the most sensibility presented the MCF-12A cell line.

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