• Beşliu Alina Institute of Microbiology and Biotechnology
  • Agafia USATÎI Institute of Microbiology and Biotechnology
  • EFREMOVA Nadejda Institute of Microbiology and Biotechnology
  • CHISELIȚĂ Natalia
Keywords: yeast, ZnO nanoparticles, Rhodosporidium toruloides, carotenoids, catalase, superoxide dismutase


The present research paper provides new information on the influence of ZnO nanoparticles (ZnO NPs) of size <50 nm and <100 nm on Rhodosporidium toruloides CNMN-Y-30 pigmented yeast. It was established that the activity of antioxidant enzymes such as catalase, superoxide dismutase and content of carotenoid pigments in the studied strain has been modified depending on the size and concentrations of NPs. There were no significant differences between the activity of antioxidant enzymes and content of carotenoid pigments in experimental group and control at the use of significantly low concentration of ZnO NPs. The use of nanoparticles in concentration of 30 mg/l caused a decrease in activity of antioxidant enzyme catalase and contributed to the increase in the activity of superoxide dismutase. This study has revealed that the concentration of 30 mg/L of ZnO NPs initiates an significant decrease in the content of carotenoid pigments - β-carotene, torulene and torularhodin in cell biomass. The results provided opportunities for modeling cell cycle processes and highlighting of carotenoid pigments and antioxidant enzymes as parameters for determining the mode of action of nanoparticles.



Aebi, H. (1984): Catalase in Vitro. Methods in Enzymology, 105, 121-126.

Aguilar-Uscanga, B., Francois, J. (2003). A study of the yeast cell wall composition and structure in response to growth conditions and mode of cultivation. Letters in Applied Microbiology, 37, 268-274.

Bernhardt, E., Colman, B, Hochella, M., Cardinale, B., Nisbet, R., Richardson, C., Yin, L. (2010). An ecological perspective on nanomaterial impacts in the environment. Journal of Environmental Quality. 39, 1954–1965, doi: 10.2134/jeq2009.0479.

Bhuvaneshwari, M., Iswarya, V., Archanaa, S., Madhu, G., Kumar, G., Nagarajan, R., Chandrasekaran, N., Mukherjee, A. (2015): Cytotoxicity of ZnO NPs towards fresh water algae Scenedesmus obliquus at low exposure concentrations in UV-C, visible and dark conditions. Aquatic Toxicology. 162, 29–38, doi: 10.1016/j.aquatox.2015.03.004.

Carocho M.,et al. A. (2013): Review on Antioxidants, Prooxidants and Related Controversy: Natural and synthetic compounds. Screening and Analysis Methodologies and Future Perspectives. Food and Chemical Toxicology, 2013, v.51, 15-25.

Cheng, T., Liang, Q., Manoor, P., Choon, N., Liya, E., Boon, H., Gyeong, H. (2017): Zinc oxide nanoparticles exhibit cytotoxicity and genotoxicity through oxidative stress responses in human lung fibroblasts and Drosophila melanogaster. International Journal of Nanomedicine, 12, 1621-1637.

Dastjerdi, R., Montazer, M. (2010): A review on the application of inorganic nano-structured materials in the modification of textiles: focus on anti-microbial proper- ties. Colloids and Surfaces B:Biointerfaces, Vol. 79, 5-18.

De Angelis, I, Barone, F, Zijno, A. (2013): Comparative study of ZnO and TiO2 nanoparticles: physicochemical characterisation and toxicological effects on human colon carcinoma cells. Nanotoxicology, 8, 1361–1372.

Efremova, N., Usatîi, A., Molodoi, E. (2013): Metodă de determinare a activității catalazei. Brevet de invenţie MD 4205, MD- BOPI nr. 2/2013.

EFSA. (2016). Safety assessment of the substance zinc oxide, nanoparticles, for use in food contact materials. Journal of EFSD. 14, 4401–4408.

El-Banna Amr A., Amal M. A. El-Razek, Ahmed R. El-Mahdy. (2012): Some Factors Affecting the Production of Carotenoids by Rhodotorula glutinis var. glutinis. Food and Nutrition Sciences, 2012, 3, 64-71.

Frengova, G., Simova, E., Grigorova, D. (1994): Formation of carotenoids by Rhodotorula glutinis in whey ultra filtrate. Biotechnology and Bioengineering, 44, 8, 288-294.

Fridovich, I. (1995): Superoxide radical and superoxide dismutases. Annu Rev Biochem. 64, 97–112.

Gunawan, C, Sirimanoonphan, A, Teoh ,WY, Marquis, CP, Amal, R. (2013): Submicron and nano formulations of titanium dioxide and zinc oxide stimulate unique cellular toxicological responses in the green microalga Chlamydomonas reinhardtii. Journal of Hazardous Materials. 260, 984-992, DOI 10.1016/j.jhazmat.2013.06.067.

Hazeem, L., Bououdina, M., Rashdan, S., Brunet, L., Slomianny, C., Boukherroub, R. (2016): Cumulative effect of zinc oxide and titanium oxide nanoparticles on growth and chlorophyll a content of Picochlorum sp. Environmental Science and Pollution Research. 23, 2821–2830, doi: 10.1007/s11356-015-5493-4.

Jaleel, C., Jayakumar, K., Chang-Xing, Z, Azooz , M. (2008): Effect of soil applied cobalt on activities of antioxidant enzymes in Arachis hypogaea. International Journal of Molecular Sciences. 3(2), 42-45. 6.

Johnson, B., Fraietta, J., Gracias, D. (2015): Acute exposure to ZnO nanoparticles induces autophagic immune cell death. Nanotoxicology. 9, 6, 737–748.

Osmond, M., Mccall, M. (2010): Zinc oxide nanoparticles in modern sunscreens: an analysis of potential exposure and hazard. Nanotoxicology. 4, 15-41, DOI 10.3109/17435390903502028.

Otero-Gonzalez, L., Garcia-Saucedo, C., Field, G., Sierra-Alvarez, R. (2013): Toxicity of TiO2, ZrO2, Fe0, Fe2O3 and Mn2O3 nanoparticles to the yeast, Saccharomyces cerevisiae. Chemosphere. 93, 1201-1206.

Prach, M, Stone, V, Proudfoot, L. (2013): Zinc oxide nanoparticles and monocytes: impact of size, charge and solubility on activation status. Toxicology and Applied Pharmacology. 266, 19–26, doi: 10.1016/j.taap.2012.10.020.

Puja K., Cynthia O., Boon H., Gyeong H. (2015): Nanotoxicity: An Interplay of Oxidative Stress, Inflammation and Cell Death. Nanomaterials. 5, 1163-1180. doi:10.3390/nano5031163

Rahman A., Nahar K., Hasanuzzaman M., Fujita M. (2016): Manganese-induced cadmium stress tolerance in rice seedlings: coordinated action of antioxidant defense, glyoxalase system and nutrient homeostasis. C R Biol. 339 462–474. 10.1016/j.crvi.2016.08.002

Sirelkhatim, A., Mahmud, S., Seeni, A., Kaus, N., Ann Ling Chuo, Bakhori,S., Hasan, H, Mohamad, D. (2015): Review on zinc oxide nanoparticles: antibacterial activity and toxicity mechanism. Nano Micro Let. 7, 219–242.

Song, W., Zhang, J., Guo, J., Zhang, J., Ding, F., Li, L., Sun, Z. ( 2010): Role of the dissolved zinc ion and reactive oxygen species in cytotoxicity of ZnO nanoparticles. Toxicology Letters. 199, 389-397, DOI 10.1016/j.toxlet.2010.10.003.

Tamas, V., Neamțu, G. (1986): Pigmenți carotenoidici și metaboliți. Ed. Ceres, București., România, vol.1, 269.

Usatîi A., Beșliu A., Chirița E. (2016): Caractere fenotipice şi compoziţia biochimică a tulpinii de levuri pigmentate Rhodotorula gracilis CNMN-Y-30. Conferința Tehnico-Ștințiifică a Colaboratorilor, Doctoranzilor și Studenților, 26 noiembrie 2015 a Univ. Tehn. A Moldovei: vol. II. Chi.: Tehnica-UTM, p. 31-35

Wahab, R., Siddiqui, M., Saquib, Q. (2014): ZnO nanoparticles induced oxidative stress and apoptosis in HepG2 and MCF-7 cancer cells and their antibacterial activity. Colloids Surf B Biointerfaces. 117, 267–276.

Zannatul, Y., Alexis, C ., Saher, M., Kelly, L., Nash, R., Glickman, D. (2016): Fabrication and Self-Assembly of Nanobiomaterials. Applications of Nanobiomaterials. Volume 1, 117-147.

Некрасова, Г., Киселева И. (2008): Экологическая физиология растений. Руководство к лабораторным и практическим занятиям. Уральский государственный университет, Екатеринбург. 157 c.

How to Cite
Alina, B., USATÎI, A., Nadejda, E., & Natalia, C. (2019). THE EFFECT OF ZnO NANOPARTICLES ON THE ACTIVITY OF ANTIOXIDANT ENZYMES AND CAROTENOID CONTENT AT RHODOSPORIDIUM TORULOIDES CNMN-Y-30 YEAST. Journal of Experimental and Molecular Biology, 19(4), 91-98. Retrieved from