EFFICACY OF CHROMOLAENA ODORATA SUPPLEMENTATION ON METHOTREXATE -INDUCED NEPHROPATHY AND OXIDATIVE STRESS

EFFICACY OF CHROMOLAENA ODORATA SUPPLEMENTATION ON METHOTREXATE -INDUCED NEPHROPATHY AND OXIDATIVE STRESS

Authors

  • Usunomena Usunobun Edo State University Uzairue
  • Bridget Ikponmwosa
  • Gabriel Anyanwu

DOI:

https://doi.org/10.47743/jemb-2024-196

Keywords:

Chromolaena odorata, Kidney, Methotrexate, nephro-protection, Oxidative stress

Abstract

We carried out this research to investigate nephro-protective efficacy of Chromolaena odorata aqueous leaf extract on kidney toxicity in Methotrexate (MTX)-induced albino male rats. Thirty (30) wistar male rats spread into five study groups were used. Group I: orally administered normal saline daily and was the normal control. Group II: received C. odorata extract (250 mg/kg B.W.) orally once daily for ten days. Group III: received MTX (7mg/kg B.W.) intraperitoneally from 8th day for three days consecutively. Group IV: received C. odorata extract (250 mg/kg B.W.) orally once daily for ten days and then administered MTX (7 mg/kg B.W.) intraperitoneally from 8th day for three days consecutively. Group V: received Vitamin C (100 mg/kg B.W.) orally once daily for ten days and then administered MTX (7 mg/kg BW) intraperitoneally from 8th day for three days consecutively. 24hours after last treatment and administration, all rats were sacrificed and blood collected in EDTA bottles for hematology or in plain tubes for biochemical analysis. Creatinine and Urea were analyzed in serum. Superoxide Dismutase (SOD), Catalase, Malondialdehyde (MDA) and histopathological analysis was assessed using the kidney tissues. The obtained results revealed that three days of consecutive Methotrexate administration caused a significant rise in kidney function activity as well as severe oxidative damage and oxidative stress shown by a significant rise in Creatinine and Urea, increased level of MDA and a significant drop in SOD and CAT. However, C. odorata attenuated Methotrexate-induced biochemical alterations and oxidative stress in kidney. Also, C. odorata alone or in combination with Methotrexate showed an increase in Packed Cell Volume (PCV), Red Blood Cells (RBC), Haemoglobin (HB), White Blood Cells (WBC) and Platelets  level compared to the group administered Methotrexate alone which showed degenerative levels in the analyzed hematological parameters. Showing corroboration, histopathological analysis showed deterioration and collapse of the kidney tubules in rats administered Methotrexate alone whereas C. odorata groups showed considerable nephro-protection and restoration towards normalcy. The results from this study revealed that the C. odorata have a nephro-protective efficacy against Methotrexate-induced kidney toxicity and damage via scavenging of free radicals and stabilization/enhancement of endogenous antioxidant status

References

Airaodion AI, Megwas AU, Ekenjoku JA, Ngwogu KO, Ngwogu AC. 2020. Nephro- and hepato-toxicity of common household insecticides used in Nigeria. International Research J Gastroenterol and Hepatol. 3(2):21-28.

APA. 2012. Guidelines for Ethical Conduct in the Care and Use of Non-Human Animals in Research. American Psychological Association (APA), Washington DC, USA. Retrieved June 13, 2015, from https://www.apa.org/science/leadership/care/careanimal-guidelines.pdf

Aladaileh SH, Omnia EH, Mohammad HA, Saghir SAM, Bin-Jumah M, Manal AA, Mousa OG, Almaiman AA, Mahmoud. AM. 2019. Formononetin Upregulates Nrf2/HO-1 Signaling and Prevents Oxidative Stress, Inflammation, and Kidney Injury in Methotrexate-Induced Rats. Antioxidants 8:430. https://doi.org/10.3390/antiox8100430

Akinmoladun AC, Akinloye O. 2007. Effect of Chromolaena odorata on hypercholesterolemia-related metabolic imbalances. Proc. Akure-Humbold Kellog/3rd SAAT Annual Conference, FUTA, Nigeria, 287-290.

Babich O, Sukhikh S, Prosekov A, Asyakina L, Ivanova S. 2020 Medicinal plants to strengthen immunity during a pandemic. Pharmaceuticals 13(10):313. https:// doi. org/ 10. 3390/ ph131 00313

Cetinkaya A, Bulbuloglu E, Kurutas EB, Kantarceken B. 2006. N-acetylcycteine ameliorates methotrexate-induced oxidative liver damage in rats. Med Sci Monit 12: 274±278.

Cohen G, Dembiec D, Marcus, J. 1970. Measurement of catalase activity in tissue extracts. Anal Biochem 34:30-38. https://doi.org/10.1016/0003-2697(70)90083-7

Dalaklioglu S, Genc GE, Aksoy NH, Akcit F, Gumuslu S. 2013. Resveratrol ameliorates methotrexate‑induced hepatotoxicity in rats via inhibition of lipid peroxidation. Hum Exp Toxicol 32:662‑71.

Dogan A. 2018. Lyophilized Extract of the Horse Mushroom, Agaricus arvensis

(Agaricomycetes), Delays Erythrocyte Hemolysis and Stabilizes Some Blood Parameters in

Carbon Tetrachloride Intoxicated Rats. Int J Med Mushrooms. 20(10):909–18.

Fawcett JK, Scott JE. 1960. A rapid precise method for the determination of urea. J Clin Path 13(2):156-9.

Iuchi TM, Akaike T, Mitsui Y, Ohshima Y, Shintani H, Azuma, et al. 2003. Glucocorticoid excess induces superoxide production in vascular endothelial cells and elicits vascular endothelial dysfunction. Circ Res 92:81–87

Ijioma S, Okafor A, Ndukuba P, Nwankwo A, Akomas S. 2014. Hypogly-cemic, hematologic and lipid profile effects of Chromolaena odorata ethanol leaf extract in alloxan-induced diabetic rats. Annals of Biol Sci 2:27–32.

Ikponmwosa BO, Usunobun U. 2022 Aqueous leaf extract of Chromolaena odorata attenuates Methotrexate-induced hepatotoxicity in wistar rats. J Fundamen Appl Pharma Sci 3(1):16-29

Lalith G. 2009. “Invasive Plants: A guide to the identification of the most invasive plants of Sri

Lanka”, Colombo, p. 116–117.

Mahi-Birjand M, Yaghoubi S, Abdollahpour-Alitappeh M, Keshtkaran Z, Bagheri N, Pirouzi A, Karimzadeh I. 2020. Protective effects of pharmacological agents against aminoglycoside-induced nephrotoxicity: A systematic review. Expert Opin. Drug Saf. 19: 167–186.

Metwall AM, Ekejinba EC. 1981. Methoxylated flavonoids and flavones from Eupatorium odoratum. Plant Medicine. 42:403 –404.

Misra HP, Fridovich I. 1972. The purification and properties of superoxide dismutase from Neurospora crassa. J Biol Chem 247:3410-3414. https://doi.org/10.1016/S0021-9258(19)45155-7

Ngozi N, Osuji T. 2014. Personal communication on the relevance and indigenous use of medicinal plants.

Odugbemi T. 2006. Outlines and pictures of medicinal plants from Nigeria. University of Lagos

Press, Lagos, Nigeria, P. 1-283.

Ohkawa H, Ohishi N, Yogi K. 1979. Assay for lipid peroxidation in animal tissues by the thiobarbituric acid reaction. Ann Biochem 95:351–358. https://doi.org/10.1016/0003-2697(79)90738-3

Ohbayashi M, Suzuki M, Yashiro Y, Fukuwaka S, Yasuda M, Kohyama N, Kobayashi Y,

Yamamoto T. 2010. Induction of pulmonary fibrosis by Methotrexate treatment in mice

lung in vivo and in vitro. J Toxicol Sci. 35(5): 653-661.

Pelvan E, Karaoğlu O, Fırat EO, Kalyon KB, Ros E, Alasalvar C. 2022. Immunomodulatory effects of selected medicinal herbs and their essential oils: a comprehensive review. J Funct Foods 94:105-108

Rofe AM, Bourgeois CS, Washington JM, Philcox JC, Coyle P. 1994. Metabolic consequences of Methotrexate therapy in tumour bearing rats. Immunol Cell Biol. 72: 43-48.

Shang ZA, Falah MA. 2019. Nickel Nanoparticles Induced Nephrotoxicity in Rats: Influence of Particle Size. Pakistan Veterinary Journal. Https://doi.org/10.29261/pakvetj/2019.106 .

Uraz S, Tahan V, Aygun C, Eren F, Unluguzel G, Yuksel M, et al. 2008. Role of ursodeoxycholic acid in prevention of methotrexate-induced liver toxicity. Dig Dis Sci 53: 1071-1077. https://doi.org/10.1007/s10620-007-9949-3 PMID: 17934844

Usunobun U, Okpiabhele A, Adegbegi AL . 2023. Telfairia occidentalis Hook f. mitigates Carbon-Tetrachloride-induced Nephrotoxicity in Wistar rats. NIPES J Sci Tech Res 5(2): 77-83

Usunobun U, Ewere GE. 2016. Phytochemical analysis, Mineral Composition and in vitro antioxidant activities of Chromolaena odorata leaves. ARC J Pharma Sci 2:6-10.

Usunobun U, Imoru NO, Ikponmwosa B, Egbo OH. 2022. Evaluation of Hepatoprotective Potential of Chromolaena odorata (L.) R.M. King & H.Rob. Against Methotrexate-induced Hepatic Toxicity in Rats. Plant Biotechnology Persa. 4(2): 1-10.

Usunobun U, Inuumoru SC, Onyeaghor C, Lawuru D, Egbo H. 2022. Nephroprotective potential of Chromolaena odorata (L.) R.M. King & H.Rob. on Methotrexate-induced Kidney damage and Oxidative stress. J Res in Appl Basic Med Sci 8(3): 118-127

Wong-Ekkabut J, Xu Z, Triampo W, Tang IM, Tieleman DP, Monticelli L. 2007. Effect of lipid peroxidation on the properties of lipid bilayers: a molecular dynamics study. Biophys J. 93:4225–4236. https://doi.org/10.1529/biophysj.107.112565

Woo CC, Kumar AP, Sethi G, Tan KHB. 2012. “Thymoquinone: potential cure for inflammatory disorders and cancer. Biochemical Pharma 83(4):443–451

Yousuf S, Shabir S, Singh MP. 2022. Protection against drug-induced liver injuries through nutraceuticals via amelioration of Nrf-2 signaling. J. Am. Nutr. Assoc., 1–21.

Zhang H, Sun S, 2015. NF-kb in inflammation and renal diseases. Cell & Biosciences. 5:63-72. Https://doi.org/10.1186/s13578-015-0056-4

Published

2024-10-09

How to Cite

Usunobun, U., Ikponmwosa, B., & Anyanwu, G. (2024). EFFICACY OF CHROMOLAENA ODORATA SUPPLEMENTATION ON METHOTREXATE -INDUCED NEPHROPATHY AND OXIDATIVE STRESS: EFFICACY OF CHROMOLAENA ODORATA SUPPLEMENTATION ON METHOTREXATE -INDUCED NEPHROPATHY AND OXIDATIVE STRESS. Journal of Experimental and Molecular Biology. https://doi.org/10.47743/jemb-2024-196

Issue

Section

Articles

Categories