SIDE COMPARATION OF TWO METHODS FOR QUANTIFYING MALONDIALDEHYDE LEVELS IN ANIMAL TISSUE EXTRACTS
Keywords:
MDA, stress marker, HPLCAbstract
Malondialdehyde (MDA) as an important marker used for the assessment of the oxidative level in a tissue of biological fluid. The standard assay method uses thiobarbituric acid for spectrophotometric detection but suffers from a lack of specificity. Here we show that an HPLC based method for quantitating the MDA has the advantages of increased sensitivity as well as a better specificity, allowing the detection of lower MDA levels in tissue extracts with increased accuracy.
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