SIDE COMPARATION OF TWO METHODS FOR QUANTIFYING MALONDIALDEHYDE LEVELS IN ANIMAL TISSUE EXTRACTS

Keywords: MDA, stress marker, HPLC

Abstract

Malondialdehyde (MDA) as an important marker used for the assessment of the oxidative level in a tissue of biological fluid. The standard assay method uses thiobarbituric acid for spectrophotometric detection but suffers from a lack of specificity. Here we show that an HPLC based method for quantitating the MDA has the advantages of increased sensitivity as well as a better specificity, allowing the detection of lower MDA levels in tissue extracts with increased accuracy.

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Published
2020-01-21
How to Cite
Roxana-Nicoleta, V.-N., & Marius, M. (2020). SIDE COMPARATION OF TWO METHODS FOR QUANTIFYING MALONDIALDEHYDE LEVELS IN ANIMAL TISSUE EXTRACTS. Journal of Experimental and Molecular Biology, 20(4), 61-66. Retrieved from http://www.jemb.bio.uaic.ro/index.php/jemb/article/view/40
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Articles