Micronucleus Test In Epithelial Cells From Oral Cavity In Koya University student Somokers And Non- Somokers
Abstract
This work aimed to investigate the use of epithelial cells from the oral cavity in identifying smoking-related effects in male smokers, normal male, normal female. To establish the relationships between micronucleated cell, binucleated cell, condensed chromatin cell. A total of 59 subjects, corresponding to 11 normal males, 19 normal female, 29 male smokers were registered for this study. The buccal epithelial cell was selected because of the direct exposure of tobacco smoke.
We appraised the incidence of micronucleus formation from 29 male smokers and who had smoked a minimum of 1 pack-year and a maximum of 12. Because of their increased smoke intake, male smokers group showed high buccal micronuclei frequency, significantly P<0.05 increased micronucleus frequency was observed in the male smokers group. Micronuclei are cytoplasmic chromatin mass with the appearance of small nuclei that arise from chromosome fragments in the anaphase stage of cell division. Their presence in cells is a reaction of structural and numerical chromosomal aberration arising during mitosis.
In an analysis of the frequency of Binucleated cell in 29 male smokers, 11 normal male statistically non-significant differences were noted. The average frequency of condensed chromatin cell in 11 normal male and 29 male smokers were high P<0.05, this is statistically significant and there is a relationship between smoking and increasing in condensed chromatin cell as we mentioned before smoking leads to cytogenetical damage to the human buccal epithelial cell.
References
Ahmad, K.K., Mustafa, S.K. and Karim, J.K., 2015. Prevalence of micronucleated cell in buccal smears among smokers and non-smokers. International Journal of Advanced Research, 3(4), pp.972-977.
Błaszczyk, E. and Mielzynska-Svach, D., 2014. Micronucleus assay in epithelial cells from the oral cavity and urinary tract in female smokers and non-smokers. Environmental Biotechnology, 10(2).pp:60-65
Błaszczyk, E. and Mielzynska-Svach, D., 2014. Micronucleus assay in epithelial cells from the oral cavity and urinary tract in female smokers and non-smokers. Environmental Biotechnology, 10.
Bonassi, S., El-Zein, R., Bolognesi, C. and Fenech, M., 2011. Micronuclei frequency in peripheral blood lymphocytes and cancer risk: evidence from human studies. Mutagenesis, 26(1), pp.93-100.
Christobher, S., Periyasamy, M., Nazeer Mohamed, B., Syed Mohamed, H.E. and Sadiq Bukhari, A., 2016 Cytogenetic Biomonitoring: Micronucleus Test in Buccal Epithelial cells of Tobacco Smokers in Tiruchirappalli District (Tamilnadu, India). Int. J. Pharm. Sci. Rev. Res., 38(1),pp:201-2005.
Diler, S.B. and Celik, A., 2011. Cytogenetic biomonitoring of carpet fabric workers using micronucleus frequency, nuclear changes, and the calculation of risk assessment by repair index in exfoliated mucosa cells. DNA and cell biology, 30(10), pp.821-827.
Fenech, M. and Bonassi, S., 2011. The effect of age, gender, diet and lifestyle on DNA damage measured using micronucleus frequency in human peripheral blood lymphocytes. Mutagenesis, 26(1), pp.43-49.
Fenech, M., 2000. The in vitro micronucleus technique. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 455(1-2), pp.81-95.
Fenech, M., 2007. Cytokinesis-block micronucleus cytome assay. Nature protocols, 2(5), p.1084.
Raj, N.S.S. and Ramdas, A., 2019. micronucleus assay of buccal mucosal cells in smokers and non-smokers. Indian Journal of Applied Research, 9(04).
Schmid, W., 1976. The micronucleus test for cytogenetic analysis. In Chemical mutagens (pp. 31-53). Springer, Boston, MA.
Suganya, R., Prabha, V., Lalitha, S. and Rajajeyakumar, M., 2019. The Role of Micronuclei as a Screening Tool in Oral Cancers. J Clin Exp Patholo, 9(368), pp.2161-0681.
Vipul Ja, P.L., Bhanu, A. and Deepak, S., 2017. Buccal cell micronuclei assay: a non-invasive genotoxic marker. IJCMR, 4(1), pp.100-4.
Yee, K.H., Jonarta, A.L. and Tandelilin, R.T., 2015. Micronucleus frequency in exfoliated buccal cells from hairdresser who expose to hair products. Dental Journal (Majalah Kedokteran Gigi), 48(2), pp.74-79.
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