A SENSITIVE METHOD FOR SALIVA DETECTION IN FORENSICS USING SALIVARY AMYLASE COUPLED WITH AMPLEX RED OXIDATION

  • Sabina Bunescu IML Iasi
  • Bogdan Alexandru Stoica IML Iasi
  • Dragos Peptanariu ICMPP Iasi
  • Liliana Foia UMF Iasi
Keywords: Forensic Science, salivary amylase, Amplex Red, saliva detection, fluorimetry, body fluids detection, serology

Abstract

A new sensitive method for saliva detection was developed, based on salivary amylase detection but with a final fluorescent product, which increases its sensitivity. After the starch is degraded due to the presence of salivary amylase, glucose is oxidised and generates hydrogen peroxide which is able to transform Amplex Red in resorufin - a highly fluorescent product. The final product is visible both under normal and UV light. The method is fast, accurate, can detect trace amounts of saliva and shows little to no interference with other body fluids. A further increase in sensitivity could be obtained by using horseradish peroxidase in the final step, but this would also lead to an increased background signal and stronger interference with urine.

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Published
2021-01-05
How to Cite
Bunescu, S., Stoica, B., Peptanariu, D., & Foia, L. (2021). A SENSITIVE METHOD FOR SALIVA DETECTION IN FORENSICS USING SALIVARY AMYLASE COUPLED WITH AMPLEX RED OXIDATION. Journal of Experimental and Molecular Biology, 21(2), 41-46. Retrieved from http://www.jemb.bio.uaic.ro/index.php/jemb/article/view/48
Section
Articles