OPTIMIZATION OF PLASMA CELL ENRICHMENT FOR CNV DETECTION BY SNP ARRAY AND MLPA
DOI:
https://doi.org/10.47743/jemb-2022-62Keywords:
Plasma Cell enrichment, SNP ARRAY, MLPA, CNVAbstract
Multiple myeloma is characterized by the uncontrolled proliferation of an atypical plasma cell clone. The main factor preventing the characterization of plasma cells is their low rate in the analyzed samples. Through this study we tried to highlight the necessity of enrichment of MM samples to be analyzed by MLPA and SNParray. Samples from 5 patients diagnosed with MM were investigated and 2 of them were enriched by magnetic sorting with anti-CD138 antibodies and analyzed by SNParray and MLPA. Unsorted samples showed a lower incidence of abnormalities. By analyzing the data, we concluded that even a 30% malignant cell infiltration in the MM sample is not enough and the magnetic sorting is a mandatory for the enrichment of target cells from the sample.
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Copyright (c) 2022 MIHAIELA LOREDANA DRAGOS, IULIU CRISTIAN IVANOV, IRINA CEZARA TRANDAFIR-VĂCĂREAN, DANIELA JITARU, MIHAELA MENȚEL, LUCIAN DRAGOȘ GORGAN
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