Assessment of the Effects of Methanol Leaf Extract of Clerodendrum violaceum on the Liver of Mice
DOI:
https://doi.org/10.47743/jemb-2023-86Keywords:
Clerodendrum violaceum, Histopathological screening, Liver function Indices, Organ-body weight ratioAbstract
Effects of methanol leaf extract of Clerodendrum violaceum on liver function indices of Swiss mice was evaluated. Six groups (A-F) of ten mice each were used. Groups B-F were administered 31.25, 62.5, 125, 250, 500 mg/kg body weight of methanol leaf extract of Clerodendrum violaceum, respectively. Group A received 5% DMSO (control). Five animals in each group were sacrificed after 14 days of administration; the remaining were sacrificed after 28 days of administration. Blood was collected for analyses, livers were collected and weighed. Some of the liver samples were homogenized and some preserved in 10% formalin for histopathological examination. After 14 days, there was significant increase (p < 0.05) in total and conjugated bilirubin and significant reduction (p < 0.05) of albumin and total protein at higher doses. Activities of ALP and γ-GT in serum were significantly elevated (p < 0.05) at all doses while liver and serum ALT activity only at lower doses. Liver and serum AST activity were also significantly elevated (p < 0.05) at higher doses. Activities of ALP and γ-GT in the liver were significantly reduced (p < 0.05) at all doses while ALT activity only reduced at the highest dose in liver and serum. AST activity was reduced at higher doses in liver but only at highest dose in serum. Liver tissue was inflamed with progressive degeneration on day 28. Results showed that methanol leaf extract of C. violaceum adversely affected the normal architecture, synthetic and secretory functions of the liver at high doses.
References
Adebayo JO, Yakubu MT, Egwim CE, Owoyele BO. 2003. Effect of ethanolic extract of Khaya senegalensis stem bark on some biochemical parameters of rat kidney. J Ethnopharmacol 88:69-72.
Adebayo JO, Balogun EA, Oyeleke SA. 2009. Toxicity study of the aqueous extract of Tithonia diversifolia leaves using selected biochemical parameters in rats. Pharmacognosy Res 1(3):143-147.
Amiragbaya F, Hapsari RA, Wulandari E. 2021. The effects of Jatropha curcas L seed extract on AST/ALT activity and the central vein thickness in liver. Pharmacogn J 13(1):66-72.
Arman M, Chowdhury KA, Bari MS, Khan MF, Ataul-Huq MM, Haque A, Capasso R. 2022. Hepatoprotective potential of selected medicinally important herbs: evidence from ethno-medicinal, toxicological, and pharmacological evaluations. Phytochem Rev. https://doi.org/10.1007/s11101-02209812-5.
Ayorinde BT, Akanji MA, Yakubu MT. 2008. Alterations in some marker enzymes of liver and kidney damage following chronic administration of aqueous extract of Tapinanthus globiferus leaves to rats. Pharmacogn Mag 4:15.
Balogun EA, Zailani AH, Adebayo JO. 2014. Augmentation of antioxidant system: Contribution to antimalarial activity of Clerodendrum violaceum leaf extract. TANG Humanitas Med. 4(4):26-1.
Caravaca-Fontán F, Azevedo L, Bayo MA, Gonzles-Candia B, Luna E, Caravaca F. 2017. High levels of both serum gamma-glutamyl transferase and alkaline phosphatase are independent predictors of mortality in patients with stage 4-5 chronic kidney disease. Nefrologia, 37:267-275.
Dillon JF, Miller MH. 2016. Gamma glutamyl transferase: “To be or not to be” a liver function test? Ann Clin Biochem 53(6):629-631.
Doumas BT, Watson WA, Biggs HG. 1971. Albumin standards and the measurement of serum albumin with bromocresol green. Clin Chim Acta 31(1):87-92.
Dutta S, Mishra PS, Sahu AK, Mishra K, Kashyap P, Sahu B. 2021. Hepatocytes and their role in metabolism. Drug Metab, 3
García-Cortés M, García-García A. 2022. Management of Pharmacologic Adverse Effects in Advanced Liver Disease. Clin Drug Investig 42 (Suppl 1), 33–38. https://doi.org/10.1007/s40261-022-01150-w
Gornall AG, Bardawill CT, David MM. 1949. Determination of serum protein by means of Biuret reaction. J Biol Chem 177:751.
Intagliata NM, Caldwell SH. 2017. Changes in liver hemostasis. J Hepatology, 67:13332-13333.
Janghel V, Patel P, Chandel SS, Vinayaka S. 2019. Plants used in the treatment of icterus (Jaundice) in Central India: A Review. Ann Hepatol, 18(5):658-672
Krause WJ. 2001. The art of examining and interpreting histologic preparations. A student handbook. Partheton Publishing group, U.K. Pp 9-10.
Levitt MD, Hapak SM, Levitt GD. 2022. Alkaline phosphatase pathophysiology with emphasis on the seldom-discussed role of defective elimination in unexplained elevations of serum ALP-A case report and literature review. Clin Exp Gastroenterol, 15:41-49.
Liao X, Li D, Ma Z, Zhang L, Zheng B, Li Z, Li G, Liu L, Zhang Z. 2022. 12-Month post-discharge liver function test abnormalities among patients with Covid-19: A single-centre prospective cohort study. Front Cell Infect Microbiol 14(12):864933, doi: 10.3389/fcimb.2022.864933.
Lilford RJ, Bentham L, Girling A. 2013. Birmingham and Lambeth Liver Evaluation Testing Strategies (BALLETS): a prospective cohort study. Health Technol Assess 17:1-307.
Nova FK. 2022. The ratio between organs weight and body weight in male black Bengal goats. Asian J Adv Res, 17(4): 11-15.
Margraf A, Lowell AC, Zarbock A. 2022. Neutrophils in acute inflammation: current concepts and translational implications. Blood 139(14):2130-2144.
Martini F, Nath JL, Bartholomeus ELF. 2012. Fundamentals of Anatomy and Physiology, 9th ed.; Person Education Inc.: San Francisco, CA, USA, pp. 890–895.
Moman RN, Gupta N, Varacallo M. 2022. Physiology, Albumin. StatPearls Publishing, Treasure Island (FL). Pg 1-2.
Moore KL, Dalley AF. 1999. Clinical oriented anatomy (4th Edition). Lippinchott Williams and Williams: A Woller Klumner Corporation, Philadelphia. Pp 287-299.
NNREC (Norwegian National Research Ethics Committee). (2019). Ethical guidelines for the use of Animals in Research. Oslo.
Nunes DR, Monteiro CS, Dos-Santos JL. 2022. Herb-induced liver injury- A challenging diagnosis. Healthcare (Basel), 10(2):278-282, doi: 10.3390/healthcare10020278.
Obeten KE, Ushie1 IE, Udoaffah G, Ndifon OO. 2017. Assessment of the effect of aqueous leaf extract of cassava (Mannihot esculenta) on adult Wistar rats. J Pharmacognosy Phytother, 9(7):117-122.
Okaiyeto K, Oguntibeju OO. 2021. African herbal medicines: Adverse effects and cytotoxic potentials with different therapeutic potentials. Int J Environ Res Public Health 18(11):5988. Doi: 10.3390/ijerph18115988.
Reitman S, Frankel S. 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28(1):56–63. Doi:10.1093/ajcp/28.1.56.
Sawieres S. 2022. Liver function tests: Indication and interpretation. Pharm J, 308(7957), Doi: 10.1211/PJ.2022.1.124202
Shi Y, Cai J, Shi C, Liu C, Zhou J, Li Z. 2022. Low serum albumin is associated with poor prognosis in patients receiving peritoneal dialysis treatment. J Healthc Eng, Article ID 7660806, https//doi.org/10.1155/2022/7660806.
Shrestha A, Neupane HC, Tamrakar KK, Bhattarai A, Katwal G. 2021. Role of liver enzymes in patients with blunt abdominal trauma to diagnose liver injury. Int J Emerg Med 14, 7. https//doi.10.1186/s12245-021-00332-1.
Sofowora A, Ogunbodede E, Onayade A. 2013. The role and place of medicinal plants in the strategies for disease prevention. Afr J Tradit Complement Altern Med AJTCAM / African Networks on Ethnomedicines. 10(1):210-229.
Szasz G, Jenoy P, Guy O. 1969. A kinetic photometric method for serum gamma transpeptidase. Clin Chem 15(2):124-136.
Ugwu CE, Suru SM. 2021. Medicinal plants with Hepatoprotective potentials against carbon tetrachloride-induced toxicity: A Review. Egypt Liver J. 11, 88, https//doi.prg/10.1186/s43066-021-00161-0
Walter M, Gerard H. 1970. Ultramicro method for the determination of conjugated and total bilirubin in serum or plasma. Microchem J 15:231–236.
Wang Y, Han T, Xue LM, Han P, Zhang QY, Huang BK, Zhang H, Ming QL, Peng W, Qin LP. 2011. Hepatotoxicity of kaurene glycosides from Xanthium strumarium L. fruits in mice. Pharmazie. 66(6):445-449.
Wright PJ, Walker S, Plummer DT. 1972. Enzymes in rat urine alkaline phosphatase. Enzymologia 42:317-327.
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